S TRANSISTOR (PNP). FEATURE. Excellent hFE linearity. MAXIMUM RATINGS (TA=25℃ unless otherwise noted). Symbol. Parameter. Value. Units. Order Number Normal Lead Free Plating S-x-TK SL-x-TK Package SOT TO Pin Details, datasheet, quote on part number: S. Part, S-TO Category. Description, LOW Voltage HIGH Current Small Signal PNP Transistor. Company, Unisonic Technologies. Datasheet, Download .
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Prepare 8550w with reverse osmosis deionized RODI or equivalently purified water. Solutions and Reagents From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: Would you like to visit your country specific website?
Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Incubate with rotation for 20 min at room temperature. Scrape cells off the plate and transfer to microcentrifuge tubes.
A cell lysate pre-clearing step is highly recommended to reduce non-specific protein binding to the Protein A Magnetic beads.
Briefly vortex the stock tube to resuspend the magnetic beads.
The optimal lysate concentration will depend on the expression level of the protein of interest. Electrotransfer to nitrocellulose membrane Protein A Magnetic Beads: Carefully remove the buffer once the solution is clear. Monoclonal antibody is produced by immunizing animals with recombinant, full-length MKK6 expressed in E. It should be noted that for the best possible results a fresh blot is always recommended.
Primary Vatasheet Dilution Buffer: Proceed to analyze by western immunoblotting or kinase activity section D.
S Datasheet PDF – Jiangsu Changjiang Electronics
Transfer supernatant containing phosphorylated substrate to another tube. This can be done by re-exposing the blot to ECL reagents and making sure there is no signal prior to adding the next primary antibody.
Dilute to 1X with dH 2 O. Separate the beads from the lysate using a magnetic separation rack, transfer the pre-cleared lysate to a clean tube, and discard the magnetic bead pellet.
Volumes are for 10 cm x 10 cm datasheett 2 of membrane; for different sized membranes, adjust volumes accordingly.
MKK6 (D31D1) Rabbit mAb #8550
Protein Blotting A general protocol for sample preparation. Pellet beads using magnetic separation rack. To harvest cells under nondenaturing conditions, remove media and rinse cells once with ice-cold 1X PBS. Biotinylated Protein Ladder Detection Pack: Wash three times for 5 min each with 15 ml of TBST.
To Purchase S View sizes. MKK3 and MKK6 are both activated by different forms of cellular stress and inflammatory cytokines 4,5.
8550S Datasheet, Equivalent, Cross Reference Search
Place tube back in magnetic separation rack. Keep on ice between washes.
Repeat washing step once more. Transfer the supernatant to a new tube. This protocol is intended for immunoprecipitation of native proteins for analysis by western immunoblot or kinase activity utilizing Protein A magnetic separation.
Incubate substrate with membrane for 1 minute, remove excess solution membrane remains wetwrap in plastic and expose to X-ray film. Reprobing of an existing membrane is a convenient means to immunoblot for multiple proteins independently when only a limited amount of sample is available. Pre-wash magnetic beads just prior to use:.
transistor S datasheet & applicatoin notes – Datasheet Archive
Changing to another country might result in loss of shopping cart. Incubate with rotation for 20 minutes at room temperature. Pre-wash magnetic beads dafasheet prior to use: Pre-wash magnetic beads see Cell Lysate Pre-Clearing section, steps 1 and 2. ATP 10 mM for kinase assays: Microcentrifuge for 5 min. The supernatant is the cell lysate. Isotype controls should be concentration matched and run alongside the primary antibody samples.
Detection of Proteins Directions for Use: Pre-clear enough lysate for test samples and isotype controls.